Search results for "Haemolytic activity"
showing 2 items of 2 documents
Cytotoxic activity of Holothuria tubulosa (Echinodermata) coelomocytes.
2017
Abstract The immune system of marine invertebrates, in particular that of holothurians, still requires further study. Our research showed that coelomocyte cells contained in the coelomic fluid of the sea cucumber, Holothuria tubulosa, are able to lyse, in vitro, red blood cells in rabbits and sheep. A plaque-forming assay showed spherule cells to be the effector cells, able to release cytotoxic molecules after xenogenic cell contact. The coelomocyte lysate supernatant, analysed by polyacrylamide gel electrophoresis overlay technique, using rabbit and sheep erythrocytes, showed two different haemolytic protein patterns: one calcium dependent and the other calcium independent. The fractions o…
Characterization of haemolytic activity of coelomocytes of Holothuria tubulosa
2013
Phylogenetic analysis recognizes echinoderms as a key group of deuterostomes, therefore the species in this group are useful for the study of the evolution of innate immunity responses. In addition, this marine invertebrate lives in coastal and estuarine waters that are directly exposed to potentially pathogenic microorganisms and stressful anthropogenic factors. Coelomocyte populations seem to be essential to immune-defence functions such as phagocytosis, ROI production, cytotoxicity, synthesis and release of antimicrobial substances including lectin, cytokine, C3-like expression, prophenoloxidase activity and capsule formation. Holothurians’ coelomocyte populations contain several coelomo…